Our enzyme for the production of glycosyltransferase stevia is non-GMO and has high transfer activity. We also comply with various overseas regulations.

Reduce the bitter taste of stevia.

Amano Enzyme supplies enzymes to produce glucosylated steviol glycosides, which is a less bitter form of stevia. Our enzymes are non-GM, organic compliant, have high glycosylation activity, and can be easily inactivated. 


Stevia is a natural sugar substitute derived from the leaf extract of the Stevia plant. It is widely used worldwide to add sweetness to foods and drinks without adding calories. The sweet taste of stevia comes from its compounds, known as steviol glycosides, which are found naturally in stevia leaves. Stevia extract has 11 main types of steviol glycosides, each with a different level of sweetness. While stevia is known to be 200 times sweeter than sugar, it can sometimes leave a bitter aftertaste1).

Steviaglycosidesweetness factor (Sugar=1)
Rebaudioside A200-400
Rebaudioside B300-350
Rebaudioside C50-120
Rebaudioside D200-300
Rebaudioside E250-300
Rebaudioside FNot available
SteviolmonosideNot available
Dulcoside A50-120

Solutions of Amano Enzyme

Sweeter taste with Amanao enzyme cyclodextrin glucanotransferase.

Stevia’s bitter taste is due to the hTAS2R4 receptor2). An enzymatic method was developed in the 1970s to reduce the bitterness of stevia. This method uses cyclodextrin glucanotransferase as the enzyme and starch or dextrin as the sugar donor to transfer the sugar to the steviosides.  These glycosylated steviosides bind less to the receptors.  Bitterness is suppressed and sweetness is improved.

The ideal balance of increased sweetness and reduced bitternes occurs with the Glycosylated steviol glycosides have 1-2 monomers of sugars attached.  Our Kleistase E5CC and β-Amylase F ‘Amano’ along with our CGT-SL can provide the optimal solution. 

Contact us to learn more.

References and Cited Papers

1) Nutrition Today 50(3):129-134, 2015
2) Phytochemistry Vol, 125, 2016, 106-111

Product List

Product Application
CGT-SL High glycosylation to stevia
KLESTASE E5CC Adjustment of glycosylation of glycosylated stevia
β-amylase F''Amano''

Application Resources

We provide a diverse collection of application materials that highlight the use of enzymes across different fields and our product offerings. Please don't hesitate to request these materials.


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